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Clone
ATCC® Number: 57198  Price: $190.00
Designation: pAL1
Depositors: R Nusse
Other Id's: GenBank:X03072
Insert Source: Homo sapiens
Insert Information: DNA: genomic
Insert lengths(kb): 2.799999952316284
Tissue: placenta
Gene product: wingless-type MMTV integration site 1, human homolog [INT1]
Biosafety Level: 1
Shipped: freeze-dried plasmid in E. coli
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Vector: Size (kb): 4.363
Vector: pBR322 (plasmid)
Type: cloning
Construction:
Marker(s):ampR,tetR
Construct size (kb): 4.363
Features: marker(s): ampR
marker(s): tetR
replicon: pMB1
Comments: Restriction digests of the clone give the following sizes (kb): EcoRI--7.2; HindIII--4.4, 2.8; AccI--3.6, 1.9, 1.6; BamHI--7.2.
A new nomenclature system has been proposed for INT1 and related genes. It has been suggested that INT1 become WNT-1. [22802]
This probe contains exons 3 and 4.
Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. [120426]
Enzyme(s) not detecting polymorphism: BamHI, EcoRI, HindIII, TaqI, MspI, BglII.
Media Description: ATCC medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
References: 8250: van Ooyen A, et al. The nucleotide sequence of the human int-1 mammary oncogene; evolutionary conservation of coding and non-coding sequences. EMBO J. 4: 2905-2909, 1985. PubMed: 2998762
11540: van't Veer LJ, et al. Molecular cloning and chromosomal assignment of the human homolog of Int-1, a mouse gene implicated in mammary tumorigenesis. Mol. Cell. Biol. 4: 2532-2534, 1984. PubMed: 6513929
22802: Nusse R, et al. A new nomenclature for int-1 and related genes: the Wnt gene family. Cell 64: 231, 1991. PubMed: 1846319
23119: Threadgill DW, et al. Syntenic conservation between humans and cattle: II. human chromosome 12. Genomics 8: 29-34, 1990. PubMed: 2081597
120426: Barbara Weiffenbach, personal communication
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