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Protozoa and Algae
ATCC® Number: 50635™  Price: $155.00
Organism: Monosiga ovata Kent
Designations: M-1
Isolation: freshwater pond, Yaroslavl, Russia, 1979
Depositors: AP Mylnikov, T Cavalier-Smith
Biosafety Level: 1
Shipped: frozen
Growth Conditions: ATCCmedium 802: Sonneborn's Paramecium medium
Temperature: 25.0°C
Protocol: ATCCNO: 50633 SPEC: The strain is routinely distributed as a frozen stabilate. Thaw the ampule and aseptically transfer the material to a T-25 tissue culture flask containing 10 ml of ATCCmedium 802. The baterial flora that is present in the shipped culture will support growth. Growth may be improved by using bacterized ATCCmedium 802 with a single species of bacteria. Bacterization is accomplished by inoculating the medium with Enterobacter aerogenes ATCC13048 approximately 24 hours before use. Other species of bacteria may work equally well but this must be empirically assessed. The culture is routinely passaged every 14 days. Although this strain does form cysts they are not stable for long periods. To subculture, agitate and aseptically transfer 0.25 ml to 10 ml of freshly bacterized medium in a T-25 flask. Screw the cap on tightly and incubate at 25C.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Classification: KINGDOM: Protozoa
References: 70312: Snell EA, et al. Hsp70 sequences indicate that choanoflagellates are closely related to animals. Curr. Biol. 11: 967-970, 2001. PubMed: 11448773
71066: Cavalier-Smith T, Chao EE-Y. Phylogeny of choanozoa, apusozoa, and other protozoa and early eukaryote megaevolution. J. Mol. Evol. 56: 540-563, 2003. PubMed: 12698292
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All prices are listed in U.S. dollars and are subject to change without notice. A discount off the current list price will be applied to most cultures for nonprofit institutions in the United States. Cultures that are ordered as test tubes or flasks will carry an additional laboratory fee. Fees for permits, shipping, and handling may apply.
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